Books+ Search Results
Yeast Metabolic Engineering Methods and Protocols
Title
Yeast Metabolic Engineering [electronic resource] : Methods and Protocols / edited by Valeria Mapelli.
ISBN
9781493905638
Publication
New York, NY : Springer New York : Imprint: Humana Press, 2014.
Physical Description
1 online resource (X, 316 p.) 44 illus., 18 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha, and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail, as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
August 31, 2018
Series
Methods in Molecular Biology, Methods and Protocols, 1152
Contents
An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae
Natural and Modified Promoters for Tailored Metabolic Engineering of the Yeast Saccharomyces cerevisiae
Tools for Genetic Engineering of the Yeast Hansenula polymorpha
Molecular Tools and Protocols for Engineering the Acid Tolerant Yeast Zygosaccharomyces bailii as a Potential Cell Factory
Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris
Methods for Efficient High Throughput Screening of Protein Expression in Recombinant Pichia pastoris Strains
Synthetic RNA Switches for Yeast Metabolic Engineering: Screening Recombinant Enzyme Libraries
Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology
Genetic Engineering of Industrial Saccharomyces cerevisiae Strains Using a Selection/Counter-Selection Approach
Evolutionary Engineering of Yeast
Determination of a Dynamic Feeding Strategy for Recombinant Pichia pastoris Strains
Yeast Metabolomics: Sample Preparation for a GC/MS-Based Analysis.- 13C-Based Metabolic Flux Analysis in Yeast: The Pichia pastoris Case
Pathway Activity Profiling (PAPi): A Tool for Metabolic Pathway Analysis
QTL Mapping by Pooled-Segregant Whole Genome Sequencing in Yeast
Genome Scale Metabolic Models of Yeast, Methods for Their Reconstruction and Other Applications
Model-Guided Identification of Gene Deletion Targets for Metabolic Engineering in Saccharomyces cerevisiae
Patents: A Tool to Bring Innovation from the Lab Bench to the Marketplace.
Subjects
Also listed under
SpringerLink (Online service)
Bookmark As
Citation
