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Zinc Finger Proteins Methods and Protocols
Title
Zinc Finger Proteins [electronic resource] : Methods and Protocols / edited by Jia Liu.
ISBN
9781493987993
Publication
New York, NY : Springer New York : Imprint: Humana Press, 2018.
Physical Description
1 online resource (XII, 276 p.) 59 illus., 44 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This detailed volume explores the notable progress in the field of zinc finger proteins (ZFP) study through widely used methods and protocols involving their biological functions and applications. Beginning with a section on the basic biology of ZFPs and design and applications of custom ZFPs, the book continues by covering methods for the evaluation and prevention of ZFN-mediated cytotoxicity as well as a collection of the representative methods of ZFN delivery. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Zinc Finger Proteins: Methods and Protocols aims to prompt both the understanding of ZFP biology and the development of next-generation ZFP therapeutics.
Variant and related titles
Springer protocols (Series)
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Printed edition:
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
September 21, 2018
Series
Methods in Molecular Biology, 1867
Contents
Part 1. Basic Biology and Engineering of ZFPs
PRDM14, a zinc finger protein, regulates cancer stemness
Computational methods for analysis of the DNA-binding preferences of Cys2His2 zinc finger proteins
Design and application of mA-specific Zinc finger proteins for the readout of DNA methylation
Enhanced manipulation of human mitochondrial DNA heteroplasmy in vitro using tunable mtZFN technology
Engineering RNA-binding proteins by modular assembly of RanBP2-type Zinc Fingers
Design of a system for monitoring ubiquitination activities of E2 enzymes using engineered RING finger proteins
Directed evolution of targeted recombinases for genome engineering
Optical recording of cellular zinc dynamics with zinc finger based biosensors
Delivery of superoxide dismutase using Cys2-His2 zinc-finger proteins
Genome editing of MSCs as a platform for cell therapy
Part 2. Methods for the Evaluation and Prevention of ZFN Cytotoxicity
Integrated multimodal evaluation of genotoxicity in ZFN-modified primary human cells
Selection and characterization of DNA aptamers against FokI nuclease domain
An improved genome engineering method using surrogate reporter-coupled suicidal ZFNs
Part 3. Delivery Methods of ZFPs
Non-transgenic approach to deliver ZFNs in seeds for targeted genome engineering
Gene editing in channel catfish via double electroporation of zinc finger nucleases
Delivery of mtZFNs into early mouse embryos
Stereotaxic surgery and viral-delivery of zinc finger epigenetic editing tools in rodent brain
In-vivo applications of cell-penetrating zinc finger transcription factors
Manufacturing and delivering genome-editing proteins.
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