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T. cruzi Infection Methods and Protocols
Title
T. cruzi Infection [electronic resource] : Methods and Protocols / edited by Karina Andrea Gómez, Carlos Andrés Buscaglia.
ISBN
9781493991488
Publication
New York, NY : Springer New York : Imprint: Humana Press, 2019.
Physical Description
1 online resource (XVII, 398 p.) 60 illus., 47 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume provides detailed descriptions on cutting-edge experimental methods to assess the multiple and complex interactions between T. cruzi and its host. Chapters detail a series of methods and protocols, ranging from rather classical biochemical/genetic approaches to modern, powerful and high-throughput ‘-omics’ technologies, to tackle this issue, as well as novel techniques to improve treatment and clinical evaluation of Chagasic patients. Genome-wide mining strategies aimed at identifying potential parasite antigens and vaccine candidates and an overview on the challenges and milestones encountered during T. cruzi genome assembly are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T. cruzi Infection: Methods and Protocols aims to provide students, researchers, and clinicians who are interested in the study of Chagas disease an indispensable source of information. .
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
March 21, 2019
Series
Methods in Molecular Biology, 1955
Contents
Trypanosoma cruzi Genome Assemblies: Challenges and Milestones of Assembling a Highly Repetitive and Complex Genome
Mining Trypanosoma cruzi Genome Sequences for Antigen Discovery and Vaccine Development
Transcriptome Studies in Trypanosoma cruzi using RNAseq
An Improved Approach to Trypanosoma cruzi Molecular Genotyping by Next-Generation Sequencing of the Mini-Exon Gene
Genome Editing by CRISPR/Cas9 in Trypanosoma cruzi
Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes
Isolation and Characterization of Extracellular Vesicles Derived from Trypanosoma cruzi
Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae
Homologous Expression of Glycosylphosphatidyl Inositol-Anchored Glycoproteins in Trypanosoma cruzi
Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi Trans-Sialidase
Exploiting Genetically Modified Dual-Reporter Strains to Monitor Experimental Trypanosoma cruzi Infections and Host: Parasite Interactions
Transmigration of Trypanosoma cruzi trypomastigotes through 3D Spheroids Mimicking Host Tissues
In Situ Detection of Dormant Trypanosoma cruzi Amastigotes using Bioluminescent-Fluorescent Reporters
Multi-Parametric Evaluation of Trypanosoma cruzi Infection Outcome in Animal Models
MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice
Genotyping of Trypanosoma cruzi from Clinical Samples by Multilocus Conventional PCR
Assessing Parasite Load in Chagas Disease Patients by Quantitative Multiplex Real-Time PCR
Diagnostic Applicability of Neutralizing Antibodies to Trypanosoma cruzi trans-sialidase
Analytics of Functional Autoantibodies in Patients with Chagas’ Disease
Apolipoprotein-A1 and Fibronectin Fragments as Markers of Cure for the Chagas Disease
Host Derived Molecules as Novel Chagas Disease Biomarkers: Hypercoagulability Markers in Plasma
Purification of GlycosylphosphatidyLinositol-Anchored Mucins from Trypanosoma cruzi Trypomastigotes and Synthesis of -Gal-Containing Neoglycoproteins: Application as Biomarkers for Reliable Diagnosis and Early Assessment of Chemotherapeutic Outcomes of Chagas Disease
Cytometric Bead Array (CBA) for Measurement Cytokines Levels in Chagas Disease Patients
Generation of T. cruzi-specific Primary CD4+ T Cell Lines from Peripheral Blood Mononuclear Cells Isolated from Chagas Disease Patients
Trypanosma cruzi Specific T-Cell Responses to Monitor Treatment Efficacy in Chronic Chagas Disease
CD8+ T Cell Response to Trypanosoma cruzi Antigens during Chronic Chagas Disease
Fluorescence Proteomic Technology to Analyze Peripheral Blood Mononuclear Cells in Chronic Chagas Disease
Isolation and Phenotypic Characterization of Inflammatory Cells from Clinical Samples: Purification of Macrophages from Trypanosoma cruzi-Infected Hearts.
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