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Medulloblastoma Methods and Protocols

Title
Medulloblastoma [electronic resource] : Methods and Protocols / edited by Abhinav Dey, Anshu Malhotra, Neha Garg.
ISBN
9781071619520
Edition
1st ed. 2022.
Publication
New York, NY : Springer US : Imprint: Humana, 2022.
Physical Description
1 online resource (X, 241 p.) 5 illus.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume details methods and protocols covering multiple aspects of Medulloblastoma. Divided into four parts, chapters guide readers through nucleic acids detection and analysis, cell-based analysis methodologies, and applications of patient-information on designing better experimental strategies for future drug development efforts in Medulloblastoma. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Medulloblastoma: Methods and Protocols aims to deliver a clear-cut and standardized set of protocols to a broad scientific community.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Printed edition:
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
February 21, 2022
Series
Methods in molecular biology ; 2423.
Methods in Molecular Biology, 2423
Contents
Childhood Medulloblastoma: An Overview
Real-time PCR assays on formalin-fixed paraffin embedded medulloblastomas
Validation of microRNA target genes using luciferase reporter assay and western blot analysis
Chromatin Immunoprecipitation Assays on Medulloblastoma Cell Line DAOY
Quantification of microRNAs in cells and tissues using stem-loop RT PCR and qPCR
Analysis of Microarray Data from Medulloblastoma tissue samples
Advancement of single- cell sequencing in Medulloblastoma
Flow cytometry approaches to obtain medulloblastoma stem cells from bulk cultures
Drug Screening assays on Medulloblastoma Stem Cells using compound libraries
3D-on-2D: A physiologically-relevant and gel-free in vitro co-culture method to assay anti-metastatic agents
In vitro screening assay for activators of T- cell migration to solid tumors
Impact of Nano preparation of phytoconstituents in Medulloblastoma
Challenges and Recent Advances of novel chemical inhibitors in Medulloblastoma Therapy
Role of ayurvedic plants as anti-cancer agents
In vivo tumorigenicity assays using subcutaneous and orthotopic xenografts in immunodeficient mice
Manufacture of an Oncolytic Measles Virus-Sensitive Immune-Competent Mouse Model of Medulloblastoma
Preclinical Research Strategy Development for RNAi-based therapies in Oncology using patient-centred information from public databases of Human Protein Atlas and R2Genomics Platform
Magnetic resonance imaging in the contemporary management of medulloblastoma: current and emerging applications
Multidisciplinary Management of Medulloblastoma: Consensus, Challenges and Controversies.
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