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Nucleic Acid Aptamers Selection, Characterization, and Application
Title
Nucleic Acid Aptamers [electronic resource] : Selection, Characterization, and Application / edited by Günter Mayer, Marcus M. Menger.
ISBN
9781071626955
Edition
2nd ed. 2023.
Publication
New York, NY : Springer US : Imprint: Humana, 2023.
Physical Description
1 online resource (XI, 282 p.) 78 illus., 66 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This updated book reflects improvements in a variety of techniques used to study the aptamer field. Beginning with a section on selection procedures, the volume continues with methods to characterize aptamers' interaction and structural properties by biophysical approaches, as well as a variety of applications that have been adapted to the aptamer compound class. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Nucleic Acid Aptamers: Selection, Characterization, and Application, Second Edition serves as an ideal guide for researchers aiming to further our understanding of aptamer biology and more.
Variant and related titles
Springer protocols (Series)
Other formats
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Printed edition:
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Format
Books / Online
Language
English
Added to Catalog
February 22, 2023
Series
Methods in Molecular Biology, 2570
Contents
Automated ssDNA SELEX Using Histidine Tagged Target Proteins
Discovery of Aptamers against Cell Surface Markers Using Ligand-Guided Selection
Implementation of Emulsion PCR for Amplification of Click-Modified DNA during SELEX
Preparation of Chemically Modified DNA Library for SELEX via Incorporation of CLB-dUTP in Primer Extension Reaction
RNA Capture-SELEX on Streptavidin Magnetic Beads
The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITE
Improvement of Aptamers by High-Throughput Sequencing of Doped-SELEX
Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry
Determination of Aptamer Structure Using Circular Dichroism Spectroscopy
Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis
Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance
Probing of Fluorogenic RNA Aptamers via Supramolecular Förster Resonance Energy Transfer with Universal Fluorescent Nucleobase Analog
Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT
Aptamer-Field-Effect Transistors for Small-Molecule Sensing in Complex Environments
Aptasensor for Impedimetric Detection of Lysozyme
Biophysical Analysis of Small Molecule Binding to Viral RNA Structures
Designing and Expressing Circular RNA Aptamers to Regulate Mammalian Cell Biology
Enzyme-Linked OligoNucleotide Assay (ELONA)
High-Throughput Development and Optimization of RNA-Based Fluorogenic Biosensors of Small Molecules Using Droplet-Based Microfluidics
Robotic APTamer-Enabled Electrochemical Reader (RAPTER) System for Automated Aptamer-Mediated Electrochemical Analysis.
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