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3D Cell Culture Methods and Protocols
Title
3D Cell Culture [electronic resource] : Methods and Protocols / edited by Zuzana Sumbalova Koledova.
ISBN
9781071636749
Edition
2nd ed. 2024.
Publication
New York, NY : Springer US : Imprint: Humana, 2024.
Physical Description
1 online resource (XIII, 337 p.) 82 illus., 77 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
Since the publication of the previous edition, the use of 3D cell and organoid cultures has become more widespread across laboratories. This second edition volume expands on the previous edition with discussions about the latest organoid models developed for many more organs; new hydrogels and devices for 3D culture; and the organoid systems that have been improved by incorporating more components of tissue microenvironments in the in vitro culture. The chapters in this book are organized into five parts and cover topics such as biofabrication, organoids, microfluidic systems, bioprinting, and image analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, 3D Cell Culture: Methods and Protocols, Second Edition is a valuable resource that will stimulate new ideas, foster interdisciplinary collaborations, and contribute to the improvement of human health and well-being.
Variant and related titles
Springer protocols (Series)
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Printed edition:
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Format
Books / Online
Language
English
Added to Catalog
March 01, 2024
Series
Methods in Molecular Biology, 2764
Contents
3D Cell Culture: Techniques For and Beyond Organoid Applications
Preparation of Corneal Tissue Matrix Bioink for 3D Bioprinting
Decellularization of Pig Lung to Yield Three-Dimensional Scaffold for Lung Tissue Engineering
Three-Dimensional Hanging Drop Spheroid Plates for Easy Chimeric Antigen Receptor (CAR) T Cytotoxicity Assay
Static 3D Osteoblast Cell Culture on 3D Printed Titanium Scaffolds
Modelling Acute Myeloid Leukaemia using StarPEG-Heparin Hydrogels
A Decision Tree to Guide Human and Mouse Mammary Organoid Model Selection
Fibroblast-Epithelium Co-Culture Methods using Epithelial Organoids and Cell Line-Derived Spheroids
Differentiation of Fibroblasts to Adipocytes in 3D for a Co-Culture with Mammary Organoids and Immunohistological Analysis
Organotypic 3D Cell Culture of the Embryonic Lacrimal Gland
Renal Organoids from Whole Kidney Cells
Organotypic 3D Ex Vivo Co-Culture Model of the Macro-Metastasis/Organ Parenchyma Interface
Addressing the Mechanical Interaction between Cancer-Associated Fibroblasts and Cancer Cells by Laser Ablation
Double-Barrel Perfusion System for Modification of Luminal Contents of Intestinal Organoids
Mechanical Actuation of Organoids in Synthetic Microenvironments
3D Breast Cancer Model on Silk Fibroin-Integrated Microfluidic Chips
Tumor-Microenvironment-on-Chip Platform for Assessing Drug Response in 3D Dynamic Culture
Suspended 3D Bioprinting for Engineering Miniaturized In Vitro Tumor Models
Real-Time Cell Cycle Imaging in a 3D Cell Culture Model of Melanoma, Quantitative Analysis, Optical Clearing, and Mathematical Modeling
Quantitative Analysis of Whole-Mount Fluorescence-Stained Tumor Spheroids in Phenotypic Drug Screens.
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