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Fluorescence In Situ Hybridization (FISH) Methods and Protocols
Title
Fluorescence In Situ Hybridization (FISH) [electronic resource] : Methods and Protocols / edited by Gal Haimovich.
ISBN
9781071637661
Edition
1st ed. 2024.
Publication
New York, NY : Springer US : Imprint: Humana, 2024.
Physical Description
XII, 302 p. 68 illus., 67 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This volume explores the latest techniques and protocols used by researchers to address unique biological questions, model organisms not typically studied by Fluorescent In Situ Hybridization (FISH), protocols combining FISH with immunofluorescence (FISH-IF), and high-throughput experiments. The chapters in this book are divided into two parts: RNA FISH protocols and DNA FISH protocols. Part One covers methods for designing OligoPaint probes and studying distinct aspects of RNA biology such as transcription and splicing dynamics, and mRNA and small RNA expression and localization. Part Two discusses DNA repair dynamics, gene compaction, and chromatin conformation and gene rearrangements in plants, insects, and mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Fluorescence In Situ Hybridization (FISH): Methods and Protocols is a valuable resource that will benefit the broader scientific community in their studies and understanding of this important field. .
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Format
Books / Online
Language
English
Added to Catalog
April 10, 2024
Series
Methods in Molecular Biology, 2784
Contents
Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in Bacteria
Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in the Fungal Pathogen Candida albicans
RNA and Protein Detection by Single-Molecule Fluorescent In Situ Hybridization (smFISH) Combined with Immunofluorescence in the Budding Yeast S. cerevisiae
Fluorescence In Situ Hybridization as a Tool for Studying the Specification and Differentiation of Cell Types in Nematostella vectensis
SABER-FISH in Hydractinia
smFISH for Plants
Fluorescent In Situ Detection of Small RNAs in Plants using sRNA-FISH
hcHCR: High-Throughput Single-Cell Imaging of RNA in Human Primary Immune Cells
High-Throughput RNA-HCR-FISH Detection of Endogenous Pre-mRNA Splice Variants
Simultaneous In Situ Detection of m6A-Modified and Unmodified RNAs using DART-FISH
Multiplexed Immunofluorescence and Single Molecule RNA Fluorescence InSitu Hybridization in Mouse Skeletal Myofibers
Designing Oligonucleotide-Based FISH Probe Sets with PaintSHOP
Efficient Prediction Model of mRNA End-to-End Distance and Conformation: Three-Dimensional RNA Illustration Program (TRIP)
Combined 3D DNA FISH, Single Molecule RNA FISH, and Immunofluorescence
Determining the Compaction State of Genes using DNA FISH
Hi-M: A Multiplex Oligopaint FISH Method to Capture Chromatin Conformations In Situ and Accompanying Open-Source Acquisition Software
Rapid DNA-FISH in Arabidopsis thaliana Somatic Cells
DBD-FISH Using Specific Chromosomal Region Probes for the Study of Cervical Carcinoma Progression
CRISPR-Based Split Luciferase as a Biosensor for Unique DNA Sequences In Situ.
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