Books+ Search Results
Flow Cytometry Protocols
Title
Flow Cytometry Protocols [electronic resource] / edited by Teresa S. Hawley, Robert G. Hawley.
ISBN
9781071637388
Edition
5th ed. 2024.
Publication
New York, NY : Springer US : Imprint: Humana, 2024.
Physical Description
XV, 458 p. 126 illus., 122 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This fifth edition volume expands on the previous editions by presenting readers with the latest developments and emerging methodologies in cytometry. The chapters in this book cover cytometry basics such as lasers for cytometry, metrics that can be used to evaluate spillover spreading, and the process of panel design and iterative optimization for spectral flow cytometry; novel methodologies such as image-enabled cell sorting, co-staining of fluorochrome-conjugated and oligonucleotide-conjugated antibodies, and screening for cell type selective probes; and a look at the achievements made in the clinical setting for both flow and mass cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Flow Cytometry Protocols, Fifth Edition is a valuable resource for researchers and scientists who are interested in continuing or expanding their knowledge of this developing field.
Variant and related titles
Springer protocols (Series)
Other formats
Printed edition:
Printed edition:
Printed edition:
Format
Books / Online
Language
English
Added to Catalog
April 10, 2024
Series
Methods in Molecular Biology, 2779
Contents
Flow Cytometry: The Glass Is Half Full
Basic Principles of Flow Cytometer Operation: The Make Your Own Flow Cytometer
Lasers Sources for Traditional and Spectral Flow Cytometry
How to Measure "Spillover Spread"
Comprehensive Immunophenotyping by Polychromatic Cytometry
Panel Design and Optimization for Full Spectrum Flow Cytometry
Practicalities of Cell Sorting
Image-Enabled Cell Sorting Using the BD CellView Technology
Monitoring Cell Proliferation by Dye Dilution: Considerations for Panel Design
Multiparametric Analysis of Apoptosis by Flow Cytometry
Quantitative and Standardized Pseudovirus Neutralization Assay for COVID-19
CRISPR-Cas9-Mediated Bioluminescent Tagging of Endogenous Proteins by Fluorescent Protein-Assisted Cell Sorting
Co-Staining with Fluorescent Antibodies and Antibody-Derived Tags for Cell Sorting Prior to CITE-seq
Discovery of Live Cell Selective Fluorescent Probesand Elucidation of Their Mechanisms: Case Study of B Cell Selective Probe CDgB
Fluorescence Lifetime Measurements and Analyses: Protocols Using Flow Cytometry and High-Throughput Microscopy
Using Artificial Intelligence to Interpret Clinical Flow Cytometry Data Sets for Automated Disease Diagnosis and/or Monitoring
Approaching Mass Cytometry Translational Studies by Experimental and Data Curation Settings
CyTOF Intracellular Cytokine Assays for Antigen-Specific T Cells
Imaging Mass Cytometry for In Situ Immune Profiling
Cytometry in High-Containment Laboratories.
Subjects
Also listed under
Bookmark As
Citation
