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ROS Signaling in Plants Methods and Protocols
Title
ROS Signaling in Plants [electronic resource] : Methods and Protocols / edited by Francisco J. Corpas, José M. Palma.
ISBN
9781071638262
Edition
1st ed. 2024.
Publication
New York, NY : Springer US : Imprint: Humana, 2024.
Physical Description
XI, 266 p. 64 illus., 46 illus. in color.
Local Notes
Access is available to the Yale community.
Access and use
Access restricted by licensing agreement.
Summary
This detailed volume presents methodologies for studying reactive oxygen species (ROS) metabolism in plant cells, including techniques for detecting different types of ROS such as superoxide radicals, hydrogen peroxide or singlet oxygen, as well as ROS generating systems such as xanthine oxidoreductase. The book provides alternatives to determine malondialdehyde, electrochemical detection approaches for determining total antioxidant capacity (TAC) as well as enzymatic antioxidants, as well as methods for studying non-enzymatic antioxidants and some components of the secondary metabolism. Written for the highly successful Methods in Molecular Biology series, the chapters in this book provide the kind of detailed implementation advice that leads to ideal results in the laboratory. Authoritative and practical, ROS Signaling in Plants: Methods and Protocols serves as an ideal guide for scientists interested in the metabolism of ROS in plant cells.
Variant and related titles
Springer protocols (Series)
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Format
Books / Online
Language
English
Added to Catalog
April 10, 2024
Series
Methods in Molecular Biology, 2798
Contents
Electrochemical Detection of Total Antioxidant Capacity (TAC) in Plant Tissues from Different Origins
Singlet Oxygen and Superoxide Anion Radical Detection by EPR Spin Trapping in Thylakoid Preparations
Singlet Oxygen Detection by Chemiluminescence Probes in Living Cells
Non-Invasive In Planta Live Measurements of H2O2 and Glutathione Redox Potential with Fluorescent roGFPs-Based Sensors
Determination of ROS Generated by Arabidopsis XANTHINE DEHYDROGENASE1 (AtXDH1) Using Nitroblue Tetrazolium (NBT) and 3,3'-Diaminobenzidine (DAP)
Malondialdehyde Assays in Higher Plants
Roles of Reactive Carbonyl Species (RCS) in Plant Response to Abiotic Stress
Measurements of Ascorbate and Dehydroascorbate in Plants Using High-Performance Liquid Chromatography
Carotenoids and Tocopherol Profiling in Fleshy Fruits
Anthocyanins: Quantification by Tandem Mass Spectrometry
Common Methods of Extraction and Determination of Phytomelatonin in Plants
Determination of Free Proline in Plants
An Enzyme Assay Kit for GABA Quantification in Plant Tissues
Identification of Superoxide Dismutase (SOD) Isozymes in Plant Tissues
Comparative Analysis of Catalase Activity in Plants: Spectrophotometry and Native PAGE Approaches
Detection of Ascorbate Peroxidase (APX) Activity in Plant Tissues: Using Non-Denaturing PAGE and Spectrophotometric Assay
Inactivation of Three Stacked Genes of Cytosolic Peroxiredoxins by Genome Editing.
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